Cor Vasa 2019, 61(3):e294-e298 | DOI: 10.33678/cor.2019.027

(Energetic metabolism of endothelial cell)

Adéla Prokopcová
Kardiologická klinika, 2. lékařská fakulta Univerzity Karlovy a Fakultní nemocnice v Motole, Praha

Endothelial cells form an active lining of vascular system. They have a very active metabolism and so they have high demand for energy. The main source of energy is glucose that is utilized by anaerobic glycolysis. Other sources are used to produce metabolic intermediates needed for the maintenance of cell structure and for signalization in resting state, and for vascular proliferation in active state. Resting endothelial cells are not very sensitive to hypoxia. A lack of glucose and coincident hypoxia lead after some time (if their supply is not restored in time) to energetic collapse and cellular necrosis.

Keywords: Aerobic glycolysis, Endothelial cell, Metabolism

Received: April 8, 2019; Accepted: May 28, 2019; Published: June 21, 2019  Show citation

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Prokopcová A. (Energetic metabolism of endothelial cell). Cor Vasa. 2019;61(3):e294-298. doi: 10.33678/cor.2019.027.
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    References

    1. Theodorou K, Boon RA. Endothelial Cell Metabolism in Atherosclerosis. Front Cell Dev Biol 2018; https://doi.org/10.3389/fcell.2018.00082 Go to original source... Go to PubMed...
    2. Eelen G, de Zeeuw P, Treos P, et al. Endothelial Cell Metabolism. Physiol Rev 2018;98:3-58. Go to original source... Go to PubMed...
    3. von Recklinghausen F. Eine Method, mikroskopische Höhle und solide Gebilde voneinander zu untersheiden. Virchow Arch 1860;19:451. Go to original source...
    4. Sumpio BE, Riley JT, Dardik A. Molecules in focus; Cells in focus: endothelial cell. Int J Biochem Cell Biol 2002;34:1508-1512. Go to original source... Go to PubMed...
    5. Cines DB, Pollak ES, Buck CA, et al. Endothelial Cells in Physiology and in the Pathophysiology of Vascular Disorders. Blood 1998;91:3527-3561.
    6. Arciniegas E, Ponce L, Hartt Y, et al. Intimal Thickening InvolvesTransdifferentiation of Embryonic Endothelial Cells. Anat Rec 2000;258:47-57. Go to original source... Go to PubMed...
    7. Štvrtinová V, Ferenčík M, Hulín I, et al. Cievny endotel ako operátor prenosu informácií medzi kardiovaskulárnym a imunitným systémom. Bratisl lek Listy 1998;99:5-19. Go to PubMed...
    8. Culic O, Gruwel ML, Schrader J. Energy turnover of vascular endothelial cells. Am J Physiol, Cell Physiology 1997;273:C205-C213. Go to original source... Go to PubMed...
    9. Oldendorf WH, Cornford ME, Brown WJ. The Large Apparent Work Capability of the Blood-brain Barrier: a study of the Mitochondrial Content of Capillary Endothelial Cells in Brain and Other Tissues of the Rat. Ann Neurol 1977;1,409-417. Go to original source... Go to PubMed...
    10. Quintero M, Colombo SL, Godfrey A, Moncada S. Mitochondria as Signaling Organelles in the Vascular Endothelium. Proc Natl Acad Sci USA 2006;103,5379-5384. Go to original source... Go to PubMed...
    11. Schoors S, Bruning U, Missiaen R, et al. Fatty Acid Carbon Is Essential for dNTP Synthesis in Endothelial Cells. Nature 2015;520,192-197. Go to original source... Go to PubMed...
    12. Huang H, Vandekeere S, Kalucka J, et al. Role of Glutamine and Interlinked Asparagine Metabolism in Vessel Formation. EMBO J 2017;36:2334-2352. Go to original source... Go to PubMed...
    13. Li JM, Shah AM. Endothelial cell superoxide generation: regulation and relevance for cardiovascular pathophysiology. Am J Physiol 2004;287:R1014-R1030. Go to original source... Go to PubMed...
    14. Ding H, Aljofan M, Triggle CR. Oxidative Stress and Increased eNOS and NADPH Oxidase Expression in Mouse Microvessel Endothelial Cells. J Cell Physiol 2007;212:682-689. Go to original source... Go to PubMed...
    15. Krutzfeldt A, Spahr R, Mertens S, et al. Metabolism of exogenous substrates by coronary endothelial cells in culture. J Mol Cell Cardiol 1990;22:1393-1404. Go to original source... Go to PubMed...
    16. Mertens S, Noll T, Spahr R, et al. Energetic response of coronary endothelial cells to hypoxia. Am J Physiol 1990;258:H689-H694. Go to original source... Go to PubMed...
    17. Peters K, Kamp G, Berz A, et al. Changes in Human Endothelial Cell Energy Metabolic Capacities during in vitro Cultivation. The Role of "Aerobic Glycolysis" and Proliferation. Cell Physiol Bioch 2009;24:483-492. Go to original source... Go to PubMed...
    18. Sweet IR, Gilbert M, Maloney E, et al. Endothelial inflammation induced by excess glucose is associated with cytosolic glucose-6-phosphate but not increased mitochondrial respiration. Diabetologia 2009;52:921-931. Go to original source... Go to PubMed...
    19. Cantelmo AR, Brajic A, Carmeliet P. Endothelial Metabolism Driving Angiogenesis: Emerging Concepts and Principles. Cancer J 2015;21:244-249. Go to original source... Go to PubMed...
    20. Carrera-Rotllan J, Estrada-Garcia L. Age-dependent changes and interrelations of number of cells and biochemical parameters (glucose, triglycerides, TBARS, calcium, phosphorus) in cultured human vein endothelial cells. Mech Ageing Dev 1998;103:13-26. Go to original source... Go to PubMed...
    21. Mann GE, Yudilevich DL, Sobrevia L. Regulation of Amino Acid and Glucose Transporters in Endothelial and Smooth Muscle Cells. Physiol Rev 2003;83:183-252. Go to original source... Go to PubMed...
    22. Lee WJ, Peterson DR, Sukowski EJ, et al. Glucose transport by isolated plasma membranes of the bovine blood-brain barrier. Am J Physiol Cell Physiol 1997;272:C1552-C1557. Go to original source... Go to PubMed...
    23. Simpson IA, Vannucci SJ, Dejoseph MR, et al. Glucose transporter asymmetries in the bovine blood-brain barrier. J Biol Chem 2001;276:12725-12729. Go to original source... Go to PubMed...
    24. Simmons RA, Flozak AS, Ogata ES. Glucose regulates GLUT-1 function and expression in fetal rat lung and muscle in vitro. Endocrinology 1993;132:2312-2318. Go to original source... Go to PubMed...
    25. Betz AL, Goldstein GW. Transport of hexoses, potassium and neutra lamino acids into capillaries isolated from bovine retina. Exp Eye Res 1980;30:593-605. Go to original source... Go to PubMed...
    26. Hutchinson ET, Eisenberg HM, Haber B. High-affinity transport ofglutamate in rat brain microvessels. Exp Neurol 1985;87:260-269. Go to original source... Go to PubMed...
    27. Hughes CCW, Lantos PL. Uptake of leucine and alanine by 299 cultured cerebral capillary endothelial cells. Brain Res 1989;480:126-132. Go to original source... Go to PubMed...
    28. Lee WJ, Hawkins RA, Peterson DR, et al. Role of oxoproline in the regulation of neutra lamino acid transport across the blood-brain barrier. J Biol Chem 1996;271:19129-19133. Go to original source... Go to PubMed...
    29. Sanchez del Pino MM, Hawkins RA, Peterson DR. Neutra lamino acid transport by the blood-brain barrier: membrane vesicle studies. J Biol Chem 1992;267:25951-25957. Go to original source...
    30. Franchi-Gazzola R, Visigalli R, Bussolati O, et al. Adaptive increase of amino acid transport system requires ERK1/2 activation. J Biol Chem 1999;274:28922-28928. Go to original source... Go to PubMed...
    31. Patella, F, Schug ZT, Persi E, et al. Proteomics-based Metabolic Modeling Reveals that Fatty Acid Oxidation (FAO) Controls Endothelial Cell (EC) Permeability. Mol Cell Proteomics 2015;14:621-634. Go to original source... Go to PubMed...
    32. Glatzel DK, Koeberle A, Pein H, et al. Acetyl-CoA Carboxylase 1 Regulates Endothelial Cell Migration by Shifting the Phospholipid Composition. J Lipid Res 2018;59:298-311. Go to original source... Go to PubMed...
    33. Wei X, Schneider JG, Shenouda SM, et al. De Novo Lipogenesis Maintains Vascular Homeostasis through Endothelial Nitric-oxide Synthase (eNOS) Palmitoylation. J Biol Chem 2011;286:2933-2945. Go to original source... Go to PubMed...
    34. Loike JD, Cao L, Brett J, et al. Hypoxia induces glucose transporter expression in endothelial cells. Am J Physiol Cell Physiol 1992;263:C326-C333. Go to original source... Go to PubMed...
    35. Dagher Z, Ruderman N, Tornheim K, et al. Acute Regulation of Fatty Acis Oxidation and AMP-Activated Protein Kinase in Human Umblical Vein Endothelial Cells. Circ Res 2001;88:1276-1282. Go to original source... Go to PubMed...
    36. Culic O, Decking UKM, Schrader J. Metabolic adaptation of endothelial cells to substrate deprivation. Am J Physiol Cell Physiol 1999;276:C1061-C1068. Go to original source... Go to PubMed...
    37. Ruderman N. B., Cacicedo J. M., Itani S., et al. Malonyl-CoA and AMP-activated protein kinase (AMPK): possible links between insulin resistance in muscle and early endothelial cell damage in diabetes. Biochem Soc Trans 2003;31:202-206. Go to original source... Go to PubMed...
    38. Artwohl M. Brunmair B, Fürnsinn C, et al. Insulin does not regulate glucose transport and metabolism in human endothelium. Eur J Clin Invest 2007;37:643-650. Go to original source... Go to PubMed...
    39. Decking UKM, Alves Ch, Wallimann T, et al. Functional aspocts of creatine kinase isoenzymes in endothelial cells. Am J Physiol Cell Physiol 2001;281:C320-C328. Go to original source... Go to PubMed...
    40. Hsu CS, Chou SY, Liang SJ, et al. Effect of physiolofic levels of glutamine on ICAM-1 expression in endothelial cells activated by preeclamptic plasma. J Reprod Med 2006;51:193-198. Go to PubMed...
    41. Yuan W, Zhang J, Li S, et al. Amine Metabolomics of Hyperglycemic Endothelial Cells Using Capillary LC-MS with Isobaric Tagging. J Proteome Res 2011;10:5242-5250. Go to original source... Go to PubMed...
    42. Watanabe H, Kuhne W, Spahr R, et al. Macromolecule permeability of coronary and aortic endothelial monolayers under energy depletion. Am J Physiol 1991;260(4 pt 2):H1344-H1352. Go to original source... Go to PubMed...
    43. Fan Y, Wu DZ, Gong YQ, et al. Effects of calycosin on the impairment of barrier function induced by hypoxia in human umbilical vein endothelial cells. Eur J Pharmacol 2003;481:33-40. Go to original source... Go to PubMed...
    44. Bierhansl L, Conradi L-C, Treps L, et al. Central Role of Metabolism in Endothelial Cell Function and Vascular Disease. Physiology 2017;32:126-140. Go to original source... Go to PubMed...
    45. Manalo DJ, Rowan A, Lavoie T, et al. Transcriptional Regulation of Vascular Endothelial Cell Responses to Hypoxia by HIF-1. Blood 2005;105:659-669. Go to original source... Go to PubMed...

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