Cor Vasa 2016, 58(6):e600-e608 | DOI: 10.1016/j.crvasa.2016.01.002

Elevated levels of miR-499 protect ischemic myocardium against uric acid in patients undergoing off-pump CABG

Fatemeh Pourrajab^a,b,*, Marzieh Sharifi^a, Seyedhossein Hekmatimoghaddam^c, Masoud Khanaghaei^a, Mahdi Rahaie^d

^a International Campus of Shahid Sadoughi University of Medical Sciences, Yazd, Iran

^b Department of Clinical Biochemistry and Molecular Biology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

^c Department of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

^d Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran

Objective: This study screens the miR-499 differences in off-pump CABG and evaluates the relationship of miR-499 with heart ejection fraction (%) and uric acid (UA) levels.

Methods and results: 70 candidates undergoing CABG were selected and left ventricular EF, blood samples and hemodynamic data were taken at different time points. Statistically significant correlation was found between circulating levels of cardiac troponin I (cTnI), UA and miR-499, after off-pump CABG (p < 0.001). Furthermore, post-operative EF was strongly affected by pre-operative miR-499 expression levels vs. UA (p < 0.005).

Conclusion: Pressure overload and ischemia result in oxidative stress (OS) on the cardiovascular system. The elevated levels of miR-499 expression in ischemic myocardium protect it from OS, while declining protective effects was observed in decreased levels of miR-499 in UA concentrations.

Keywords: Ischemia; Mechanism; MiR-499; Uric acid

Received: October 13, 2015; Revised: January 6, 2016; Accepted: January 7, 2016; Published: December 1, 2016  Show citation