Cor Vasa 2007, 49(10):356-361 | DOI: 10.33678/cor.2007.128

Heat shock proteins Hsp60 and Hsp70 in cardiac surgical patients

Karolína Jankovičová¹, Manuela Kudlová¹, Martina Koláčková¹, Pavel Kuneš^1,2, Ctirad Andrýs¹, Jiří Manďák², Vladimír Lonský², Jan Krejsek¹

¹ Ústav klinické imunologie a alergologie

² Kardiochirurgická klinika, Univerzita Karlova v Praze, Lékařská fakulta a Fakultní nemocnice Hradec Králové, Hradec Králové, Česká republika

Background/Aims:
To study the perioperative and postoperative serum levels of heat shock proteins Hsp60 and Hsp70 in cardiac surgical patients operated with or without cardiopulmonary bypass (CPB).

Methods:
Thirty-four patients undergoing coronary artery bypass grafting (CABG) were operated either with or without the use of CPB. The subgroups did not differ in age, sex, number of anastomoses, and ejection fraction. Samples of venous blood were taken at five intervals: at the induction to anesthesia, upon completion of surgery and on postoperative days 1, 3, and 7. Hsp60 and Hsp70 levels were detected by commercially available ELISA kits.

Results:
Hsp60 levels did not differ between patients with or without CPB. Hsp60 levels were significantly decreased in patients operated without CPB on postoperative day 7 (p < 0.0151). The dynamics of Hsp70 was different between the subgroups with and without CPB. We found significantly increased levels of Hsp70 upon completion of surgery in patients operated with CPB (p < 0.0259).

Conclusion:
The study demonstrated increased Hsp70 levels in cardiac surgical patients undergoing CABG using CPB compared with patients operated on the beating heart. This finding is in accordance with more profound ischemia-reperfusion injury in patients operated with the use of CPB.

Keywords: Cardiac surgery; Hsp60; Hsp70; CPB (cardiopulmonary bypass)

Published: October 1, 2007  Show citation